This laboratory focuses on finding, clarification, and application of superior microbial systems. These research subjects are carried out by molecular biology (functional analysis of genes and enzymes/proteins), cell biology (electron and fluorescence microscopy), structural biology (X-ray crystallography), and synthetic biology (metabolite and gene-expression analysis) including omics biology (genomics, transcriptomics, proteomics, metabolomics).
The recent research subjects are as follows:
1. Microbial systems for interaction with animal (human) and plant
Sayoko Oiki, Yusuke Nakamichi, Yukie Maruyama, Bunzo Mikami, Kousaku Murata, and Wataru Hashimoto. Streptococcal phosphotransferase system imports unsaturated hyaluronan disaccharide derived from host extracellular matrices.
PLoS ONE,14(11), e0224753(2019).
2. Dynamics in microbial cell membrane
Wataru Hashimoto, Yukiko Miyamoto, Mayumi Yamamoto, Fuminori Yoneyama, and Kousaku Murata. A novel bleb-dependent polysaccharide export system in the nitrogen-fixing Azotobacter vinelandii subjected to low nitrogen gas levels.
Int. Microbiol., 16(1), 35-44 (2013).
3. Microbial systems for utilization of macromolecules
Hidenori Konishi, Mamoru Hio, Masahiro Kobayashi, Ryuichi Takase, and Wataru Hashimoto. Bacterial chemotaxis toward polysaccharide pectin by pectin-binding protein.
Sci. Rep., 10(1), 3977 (2020).
4. Microbial systems for utilization of metal proteins
Sakiko Nakatsuji, Kenji Okumura, Ryuichi Takase, Daisuke Watanabe, Bunzo Mikami, and Wataru Hashimoto. Crystal structures of EfeB and EfeO in a bacterial siderophore-independent iron transport system.
Biochem. Biophys. Res. Commun., 594, 124-130 (2022).
5. Microbial production of useful materials
Shota Nakata, Mamoru Hio, Ryuichi Takase, Shigeyuki Kawai, Daisuke Watanabe, and Wataru Hashimoto. Polyunsaturated fatty acids-enriched lipid from reduced sugar alcohol mannitol by marine yeast Rhodosporidiobolus fluvialis Y2.
Biochem. Biophys. Res. Commun., 526(4), 1138-1142 (2020).