This laboratory focuses on finding, clarification, and application of superior microbial systems. These research subjects are carried out by molecular biology (functional analysis of genes and enzymes/proteins), cell biology (electron and fluorescence microscopy), structural biology (X-ray crystallography), and synthetic biology (metabolite and gene-expression analysis) including omics biology (genomics, transcriptomics, proteomics, metabolomics).
The recent research subjects are as follows:
1. Microbial systems for interaction with animal (human) and plant
Shun Yabuuchi, Sayoko Oiki, Shuma Minami, Ryuichi Takase, Daisuke Watanabe, and Wataru Hashimoto. Enhanced propagation of Granulicatella adiacens from human oral microbiota by hyaluronan.
Sci. Rep.,12(1), 10948(2022).
Sayoko Oiki, Yusuke Nakamichi, Yukie Maruyama, Bunzo Mikami, Kousaku Murata, and Wataru Hashimoto. Streptococcal phosphotransferase system imports unsaturated hyaluronan disaccharide derived from host extracellular matrices.
PLoS ONE,14(11), e0224753(2019).
2. Dynamics in microbial cell membrane
Haruka Sugiura, Ayumi Nagase, Sayoko Oiki, Bunzo Mikami, Daisuke Watanabe, and Wataru Hashimoto. Bacterial inducible expression of plant cell wall-binding protein YesO through conflict between Glycine max and saprophytic Bacillus subtilis.
Sci. Rep., 10(1), 18619 (2020).
Wataru Hashimoto, Yukiko Miyamoto, Mayumi Yamamoto, Fuminori Yoneyama, and Kousaku Murata. A novel bleb-dependent polysaccharide export system in the nitrogen-fixing Azotobacter vinelandii subjected to low nitrogen gas levels.
Int. Microbiol., 16(1), 35-44 (2013).
3. Microbial systems for utilization of macromolecules
Kotaro Anamizu, Ryuichi Takase, Mamoru Hio, Daisuke Watanabe, Bunzo Mikami, and Wataru Hashimoto. Substrate size-dependent conformational changes of bacterial pectin-binding protein crucial for chemotaxis and assimilation.
Sci. Rep., 12(1), 12653 (2022).
Hidenori Konishi, Mamoru Hio, Masahiro Kobayashi, Ryuichi Takase, and Wataru Hashimoto. Bacterial chemotaxis toward polysaccharide pectin by pectin-binding protein.
Sci. Rep., 10(1), 3977 (2020).
4. Microbial systems for utilization of metal proteins
Sakiko Nakatsuji, Kenji Okumura, Ryuichi Takase, Daisuke Watanabe, Bunzo Mikami, and Wataru Hashimoto. Crystal structures of EfeB and EfeO in a bacterial siderophore-independent iron transport system.
Biochem. Biophys. Res. Commun., 594, 124-130 (2022).
Kanate Temtrirath, Kenji Okumura, Yukie Maruyama, Bunzo Mikami, Kousaku Murata, and Wataru Hashimoto. Binding mode of metal ions to the bacterial iron import protein EfeO.
Biochem. Biophys. Res. Commun., 493(2), 1095-1101 (2017).
5. Microbial production of useful materials
Nobuhiro Yoshida, Ryuichi Takase, Yoshimi Sugahara, Yuko Nambu, and Wataru Hashimoto. Direct production of polyhydroxybutyrate and alginate from crude glycerol by Azotobacter vinelandii using atmospheric nitrogen.
Sci. Rep., 12(1), 8032 (2022).
Shota Nakata, Mamoru Hio, Ryuichi Takase, Shigeyuki Kawai, Daisuke Watanabe, and Wataru Hashimoto. Polyunsaturated fatty acids-enriched lipid from reduced sugar alcohol mannitol by marine yeast Rhodosporidiobolus fluvialis Y2.
Biochem. Biophys. Res. Commun., 526(4), 1138-1142 (2020).