This laboratory focuses on finding, clarification, and application of superior microbial systems. These research subjects are carried out by molecular biology (functional analysis of genes and enzymes/proteins), cell biology (electron and fluorescence microscopy), structural biology (X-ray crystallography), and synthetic biology (metabolite and gene-expression analysis) including omics biology (genomics, transcriptomics, proteomics, metabolomics).
The recent research subjects are as follows:
1. Microbial systems for interaction with animal (human) and plant
Yukie Maruyama, Sayoko Oiki, Ryuichi Takase, Bunzo Mikami, Kousaku Murata, and Wataru Hashimoto: Metabolic fate of unsaturated glucuronic/iduronic acids from glycosaminoglycans: Molecular identification and structure determination of streptococcal isomerase and dehydrogenase.
J. Biol. Chem., 290(10), 6281-6292 (2015).
2. Microbial systems for utilization of macromolecules
Yukie Maruyama, Takafumi Itoh, Ai Kaneko, Yu Nishitani, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Structure of a bacterial ABC transporter involved in the import of the acidic polysaccharide alginate.
Structure, 23(9), 1643-1654 (2015).
3. Microbial systems for utilization of metal proteins
Wataru Hashimoto, Akihito Ochiai, Chang Soo Hong, Kousaku Murata, and Ananda M. Chakrabarty: Structural studies on Laz, a promiscuous anticancer Neisserial protein.
Bioengineered, 6(3), 141-148 (2015).
4. Microbial production of useful materials
Fuminori Yoneyama, Mayumi Yamamoto, Wataru Hashimoto, and Kousaku Murata: Production of polyhydroxybutyrate and alginate from glycerol by Azotobacter vinelandii under nitrogen-free conditions.
Bioengineered, 6(4), 209-217 (2015).
5. Molecular biology and applied microbiology of NADP+
Yutaka Kawabata, Kousaku Murata, and Shigeyuki Kawai: Significance of Ser-188 in human mitochondrial NAD kinase as determined by phosphomimetic and phosphoresistant amino-acid substitutions.
Biochem. Biophys. Res. Commun., 468, 691-695 (2015).